Scientific Journal

Scientific Journal of the Hellenic Companion Animal Veterinary Society (HCAVS)


Evaluation of toxicity of a silver-nanoparticle cream in healthy rats’ skin

Kouki M.I. PLiN Nanotechnology A.Ε., 57001, Thessaloniki, Greece
Tsouknidas A. PLiN Nanotechnology A.Ε., 57001, Thessaloniki, Greece
Vlachou A. PLiN Nanotechnology A.Ε., 57001, Thessaloniki, Greece
Kaldeli-Kerou A. PLiN Nanotechnology A.Ε., 57001, Thessaloniki, Greece
Karamanidou Th. PLiN Nanotechnology A.Ε., 57001, Thessaloniki, Greece
Brellou G. Associate Professor, Laboratory of Pathology, School of Veterinary Medicine, Aristotle University, Thessaloniki, Greece
Lampropoulou D. Associate Professor, Department of Chemistry, Environmental Pollution Control Laboratory, Aristotle University, Thessaloniki, Greece
Papadimitriou S.A. Professor, Companion Animal Clinic, School of Veterinary Medicine, Aristotle University Thessaloniki, Greece


In the present study, silver nanoparticles (AgNPs) accumulation and potential histopathological changes in the skin of rats were evaluated after chronic application as a cream.

Materials and methods

Twenty Wistar rats were included. Animals were shaved on both sides of the spine and a 10 ppm AgNP cream was applied on one side for 14 days. A cream containing the excipients was applied on the other side. The skin was evaluated daily and any macroscopic side effects were recorded. Finally, the animals were sacrificed and 2 specimens from each side were obtained for histopathological and toxicity evaluation.


None of the animals exhibited pathological signs. Histopathology revealed minor changes including foci with hyperkeratosis, as well as intracellular and sparsely intercellular edema of stratum spinosum. Dermis showed focal interstitial edema, infiltration by mononuclear cells, and hair follicle dilatation. The toxicological study of the samples revealed the presence of silver with a mean value of 1.359 μg/gr.


The proposed AgNPs cream does not inflict significant pathological changes on the skin of rats, while their accumulation, probably in the hair follicles may result in a prolonged beneficial effect on the skin and hair of the animals.

The results and experiments were carried out within the framework of the nanovet project (T2EDK - 02826) by EU ERDF and Greek funds through the Operational Program Competitiveness, Entrepreneurship and Innovation.


  • Fang CL, Aljuffali IA, Li YC, Fang JY. Delivery and targeting of nanoparticles into hair follicles. Ther Deliv. 2014;5(9):991-1006.
  • Samberg ME, Oldenburg SJ, Monteiro-Riviere NA (2010) Evaluation of silver nanoparticle toxicity in skin in vivo and keratinocytes in vitro. Environmental Health Perspectives. Mar, 118(3),407-413.
  • Zhang XF, Liu ZG, Shen W & Gurunathan S (2016) Silver Nanoparticles: Synthesis, Characterization, Properties, Applications, and Therapeu- tic Approaches. Int J Mol Sci. 17, 1534.

Quick Links

Social Media

Contact us

Hellenic Journal of
Companion Animal Medicine

Apollo Tower
64 Louizis Riankour Street,
115 23 Athens
Tel.: +30 210 7759727
Fax.: +30 210 7753460