Pappa Ε. Ι. DVM, PhD student, Department of Veterinary Clinics Study, Faculty of Veterinary Science, University of Thessaly, Karditsa, Greece
Athanasiou L. V. DVM, PhD, Professor, Department of Veterinary Clinics Study, Faculty of Veterinary Science, University of Thessaly, Karditsa, Greece
Georgiou S. DVM, PhD, Adjunct teaching staff, Department of Veterinary Clinics Study, Faculty of Veterinary Science, University of Thessaly, Karditsa, Greece
Barbagianni M. DVM, PhD, Assistant Professor, Department of Veterinary Clinics Study, Faculty of Veterinary Science, University of Thessaly, Karditsa, Greece
Psalla D. DVM, PhD, Associate Professor, Laboratory of Pathology, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, Greece
Fthenakis G. DVM, PhD, Professor, Department of Veterinary Clinics Study, Faculty of Veterinary Science, University of Thessaly, Karditsa, Greece
Tsioli V. DVM, PhD, Associate Professor, Laboratory of Pathology, School of Veterinary Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, Greece
Sideri A. DVM, PhD, Assistant Professor, Department of Veterinary Clinics Study, Faculty of Veterinary Science, University of Thessaly, Karditsa, Greece
Introduction
Recent research in bone healing has focused on biomaterials. Stromal Vascular Fraction (SVF) possesses osteogenic, osteoinductive, immunomodulatory, anti-inflammatory, and angiogenic properties. Research on the use of autologous SVF for bone healing has produced encouraging results. The objective of this study was the evaluation of SVF use for the augmentation of the healing process in a large animal model.
Materials and methods
Bone defect was created on the metatarsus of 24 healthy sheep. The defect was filled with Hydroxyapatite (HA) bone paste (Group A, n=6 sheep), autogenous bone graft mixed with HA (B, n=6), SVF mixed with HA (C, n=6), and a combination of all the above biomaterials (D, n=6). Each animal was evaluated by clinical examination and imaging at regular intervals for 90 days. On day 90, biopsy was performed.Statistical analysis included analysis of variance and analysis of covariance. Statistical significance was set at p<0.05.
Results
SVF was successfully isolated. On D90 animals in Group C were found with best radiological (p=0.003) and vascularization scores (p=0.02).Ultrasonographic length of bone defect was shortest in Group C (p=0.0006) and histologic analysis revealed the best healing score (p=0.015).
Conclusions
SVF isolation was found to be an easy and effective technique for long bone healing and might be potentially used as an alternative to bone grafting.
References
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